Course Description

• Course name and number:Animal and plant tissue culture Bio 474
• Course meeting times, places:
• Bio 474 Sunday and Tuesday11-12Room: 1167
• Course website address:
• Course prerequisites and requirementsBIO 202
• Description of the course (what, why, philosophy, teaching methodology)
• The course is going to be taught in English video clips and media tools are going to be used to make understanding easier for students. Interactive quiz will be given to the student by the end of each chapter. Students will be told to subscribe to the website related to the text book so that they can use all the facilities provided for better learning.

Course Goals & Objective

• A statement of what the student will know and be able to do as the result of learning

   

  The course explores history of cell and tissue culture - basis of cell and tissue culture - culture media – component preparation – methods of media sterilization – preparation of different tissues – micropropagation of plants – growth and development of animal embryos – protoplast culture – cryopreservation – synthetic seed – somaclonal variation – organ transportation in animals – stem cells and artificial organs- animal cloning. Research and applications of animal cell culture – differentiation of animal cells – animal cell culture techniques – regulation of gene expression and transcriptional control.

   

• Knowledge and Understanding:

• Understand basic of Cell Culture: Historical Background
• Importance of and progress in Cell Culture Technology
• Basic requirement for growing animal cells, factors effected for growing animal cells, Cell a8) preservation Cellular Interactions
• Technology involved in cell and tissue culture.
• Structure, growth and function of plant and animal cells
• Plant cell technology, In vitro conservation, Protoplast culture; micropropagation
• Cell cloning and fusion Hybridoma technology
• Applications of stem cells for agriculture and therapeutic purposes.

   

  • b) Intellectual Skills

  • to provide training in the design and use of cell culture facilities
  • to develop experience in basic cell culture techniques.
  • to provide an understanding of cell culture, its uses and applications
  • to develop observational and record keeping skills
  • to develop a range of transferable skills

   

   

  • c- Professional and Practical Skills

This course will teach and train the student on the methods of cell and tissue culture using the latest available methods in order to be able to apply it in the different practical aspects of life.

d- General and Transferable Skills

Upon completing the requirements of this course student should:

·Becomefamiliar with terms used in plant and animal

·Discuss the uses of Biotechnology in plants and animal

·Perform micro propagation and plant tissue culture

·Become familiar with terms used in animal Biotechnology

·Discuss the uses of Biotechnology in animals

·Identify the applications of animal Biotechnology

Learning Resources

• Student assessment:A clear rationale and policy on grading

  First exam 15marks

  Mid-Term exam 15 marks

  Attendance 5 marks

  Lab 25 marks

  Final Exam 40 marks

• Expectations from students: Attitudes, involvement, behaviors, skills, and ethics

  Students have to respect University rules and ethics, and show good behavior. Absence of 25% without medical report might forbid the student from attending the final exam.

• Student responsibilities to the course

  Students have to do the assignments and attend lectures and exams.

• Expectations for each assignment and project
• Important rules of academic conduct

  Students have to respect the time of lecture, listen to professors, propose question and learn about the different subjects of the course. Student must understand the meaning of scientific theft.

   

• Lab plan and assignments, if it applies

• Students must acquire information of cell culture including methods of animal and Plant Tissue Culture and Regeneration Overview
  • Agrobacterium tumefaciens mediated transformation
  • Animal tissue culture fundamentals: growth conditions, biological safety
  • cabinets, and media, adherent vs. suspension cultures, tissue culture formats
  • Tissue culture contaminants focusing on mycoplasma
  • Primary tissue cell culture; cloning schemes and options for picking clones
  • Transfections; hybridomas; cryopreservation

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